Monoclonal
Abs - 4
Recombinant DNA technique:
emRNA is isolated from lymphoid tissue of
donor who has been
sensitized with a specific antigen
eisolated mRNA is converted to dsDNA using
reverse transcriptase
+ DNA polymerase enzymes
epolymerase chain reaction (PCR) is used to
amplify the gene fragments
of VH and VL
ethe amplified fragments of VH and VL are
then injected into E.
coli bacteriophage via a plasmid vector and gene fragments fuse with bacteriophage protein
eeach recombinant bacteriophage contains a
different combination
of VH and VL genes
thus producing antibodies of differing specificities.
ethese specificities are then identified by
reacting with different
antigens and are then cataloged into a monoclonal antibody library
