Use
of Restriction Enzymes
Ubacterial
restriction enzymes (called endonucleases) have the ability to recognize short DNA base pair sequences
and cleave the covalent
bonds between adjacent nucleotides at a specific recognition site resulting in 4-8
recognition sequences
Uthese
restriction enzymes are named for the bacteria that produces them:
Eco RI =
Hind III =
Eco =
Escherichia coli
R = RY13 strain
I = 1st
nuclease to be isolated
Hin =
Haemophilus influenzae
d = Rd strain
III = 3rd
nuclease to be isolated
