Hybridization
Assays
allow fragments
of known nucleic acid composition (called a Aprobe@)
to locate the matching (complementary) sequence in unknown sample
the denatured
(seperated) DNA strands can be reformed (annealed) to produce a double-stranded DNA
when one of the
DNA strands is labelled with a signal-emitting tag such as:
radioisotopes:
32P,
35S, or 125I
enzymes:
alkaline
phosphatase or peroxidase
biotin + avidin
+ enzyme
biotin + avidin
+ fluorochrome
