Types
of Hybridization Assays - 1
Southern
blot
Restriction
Fragment Length Polymerization (RFLP)
Dot Blot
Hybridization
E used to measure DNA
E combines electrophoretic separation with
hybridization procedure
E used to determine polymorphism of HLA alleles
and identification of an abnormal gene
E also called immunoblot
E sample DNA immobilized in solid phase such as:
cellulose or
nylon membrane
E allows for multiple samples/membrane
