NASBA
Method
eNucleic Acid Sequence-Based
Amplification (NASBA)
E used to amplify target RNA
E employs three enzymes:
]reverse
transcriptase:
]ribonuclease
H:
]T7 RNA
polymerase:
-makes complementary DNA strand from RNA
and it serves as
template for rurther replication
-degrades RNA
to allow for a complete-double stranded DNA
-produces
multiple copies of RNA from DNA template
clinical
application:
detection of
viral RNA
