Molecular
Fingerprinting - 2
Application:
etechniques used for the detection of
polymorphism of HLA alleles
Molecular techniques:
eRestriction fragment length polymorphism
(RFLP)
esequence-specific priming (SSP)
esequence-specific oligonucleotide probing
(SSOP)
esequence-base typing (SBT)
uses Southern blotting technique
identifies HLA loci that code for individual
HLA antigens
alternative name = Amolecular fingerprinting@
uses PCR/gel electrophoresis technique
detects polymorphism at allele level
uses PCR/hybridization probes to PCR
products
detects HLA alleles
uses PCR/nucleotide sequencing of PCR
products
detects alleles at exact sequence level
