Notes

Slide Show

Outline

1	CLSC320 Principles of Immunology Immunohematology Applications Program for Clinical Laboratory Science Unit - 16 Blood Banking Reagents
2	Unit – 16 Guidelines Reading assignment: Study unit on Instructor’s website www.andrews.edu/AHLE/awm Learning objectives: 2. Compare and contrast polyclonal and monoclonal antisera.
3	Unit – 16 Guidelines Learning objectives: continued 7. Discuss the different types and purposes of reagent red blood cells.
4	Polyspecific vs Monospecific Antisera Polyspecific antisera: Examples of Blood Bank antisera:
5	Anti-A & Anti-B
6	Antisera for ABO Forward Typing
7	Anti-A, B
8	ABO Antigens & Antibodies
9	Anti-D and Rh Control
10	Rh antisera
11	Rh Control Type of control: Required when anti-D antisera has macromolecules in a high-protein diluent to enhance agglutination.
12	Antiglobulin Reagents Polyspecific Anti-human globulin (AHG)
13	Anti-Human Globulin (AHG)
14	Polyspecific AHG What is in it: Used when performing Direct Antiglobulin Testing (DAT) on adults.
15	Monospecific AHG What is in it: Used when positive Direct Antiglobulin Testing (DAT) on adults to identify antibody involved.
16	Check Cells
17	Check Cells Alternate name: Red blood cells that have anti-IgG on surface
18	Reagents Red Blood Cells A₁ cells
19	A₁ and B cells
20	Reverse ABO Typing
21	Antibody Screening Cells
22	Screening Cells Either two or three vials containing red blood cells from group O donors.
23	Antibody Panel Cells
24	Panel Cells What is in it: Used when positive Antibody Screening Test (ASC) has been obtained and is used to identify antibody(ies) present.
25
26	Lectins Extracts of seeds that act like antibodies and have specificities to certain red blood cell antigens and will cause them to agglutinate.
27	Gel Technology When was it developed: Gel suspended anti-IgG contained in pre-filled plastic cards
28	Gel Technology
29	Gel Technology
30	Gel Technology
31	Gel Technology
32	Microplate Technology
33	Solid-Phase Red Blood Cell Adhesion Technology
34	Quality Control of Reagents
35	Ortho Confidence Program
36	End – Blood Bank Reagents