Reverse ABO Typing
•Reagents used:
•A1 cells – in a 2-5% suspension and negative for D, C, E
•Used to detect presence of anti-A1 in plasma
•B cells – in a 2-5% suspension and negative for D, C, E
•Used to detect presence of anti-B in plasma
A1 cells
B cells
ABO Blood Type
+
0
+
0
0
0
+
+
B
A
O
AB
+ = agglutination of red cells
0 = no agglutination of red cells