Solid-Phase Red Blood Cell Adhesion Technology
Microtiter plates have wells
coated with antigen and plasma from
patient added. Patient antibody will attach to antigen if specificity matches. Wells are washed to remove unbound immunoglobulins. Red cells coated with anti-IgG
(indicator cells) are added and plate is centrifuged.
Principle:
Negative test:
Interpretation of results:
Red cells all
on bottom of well
Positive test:
Red cells on
bottom of well and on sides of wells.
Detection of specific IgG
antibodies in plasma of patients.
Applications: