Coagulation Testing-Principle
Kinetic assay:
“assays that form increasing amounts of products with time”
Temperature:
Factors
that affect kinetic assays:
pH:
Substrate
concentration:
Doubling of rate for each 10oC
rise
Each enzyme has optimum range
Lower the concentration the slower the rate of
reaction
Principle
of coagulation studies:
The temperature is maintained at 37oC
and the pH is maintained by use of buffers so that the only factors influencing product formation (clot)
are the amount of substrate present (clotting factors) or the amount of inhibitors (heparin or coumadin)
present.