Hemoglobin Determination - 1
Method most commonly used:
Name of
reagent:
Cyanmethemoglobin
assay
Principle of
assay:
Sodium
bicarbonate:
Potassium
cyanide (KCN)
Drabkin’s
reagent
Lysis
RBCs
Potassium
ferricyanide (K3Fe(CN)6)
Converts
hemoglobins to methemoglobin
(Fe++ to Fe+++)
(Fe++ to Fe+++)
Combines
with methemoglobin to form cyanmethemoglobin