Hemoglobin Determination - 2
Wavelength used to read cyanmethemoglobin:
Relationship of absorbance to concentration:
540 nm
Which hemoglobins are measured by this method:
All hemoglobins except sulfhemoglobin
Absorbance is directly proportional to hemoglobin concentration
How are absorbances converted to g/dL of hemoglobin:
Using hemoglobin Standard curve
Cunknown = Cstandard x Aunknown 
                             Astandard
or