Hemoglobin Std Curve - 1
Steps involved in making Std Curve:
1. Make dilutions of a 20 g/dL hb std
2. Mix each dilution with Drabkin’s
3. Measure absorbance of each
dilution
4. Plot absorbance of each dilution:
Linear
paper =
Semilog
paper =
Strait line
Curved line
5. Plot % Transmittance of each
dilution:
Linear
paper =
Curved line
Semilog
paper =
Strait line
6. Using either standard curve
convert patient
absorbance or %T to g/dL hemoglobin