CLSC411

Blood Smear Staining - 1

Stains used :

Wright’s stain:

eosin

Wright-Giemsa stain:

Methylene blue

Methylene blue azure

Procedure for staining :

1. Dip in absolute methanol to fix blood to slide

2. Add stain

Methylene blue

3. Add buffer to green sheen (pH 6.4 – 6.8)

4. Rinse with distilled water

5. Air dry rapidly

Cover slip method : click here to see method

Gives best distribution of leukocytes

Only used for bone marrow aspirates – hard to handle