Blood Smear Staining - 2
Cellular components
stain :
RNA:
Stained
by methylene blue (basophilic) light blue
Hb:
Stained
by methylene blue (basophilic) deep blue
DNA:
Gray
colored RBCs & eosinophils & dark WBCs:
Buffer
too alkaline
Stained
by eosin (acidic) reddish - pink
Problems with stain :
Rinsed
too short a time
Stained
too long a time
Heparin
used as anticoagulant