Direct Antiglobulin Test (DAT)
Principle:
Erythrocytes
with antibodies attached will agglutinate when antihuman
globulin is added to washed cell
suspension.
Clinical application:
antibody sensitized red cells
return
Procedure:
3-5% suspension of patient’s red cells are washed
three times
2 drops of antihuman globulin added, mixed,
and centrifuged
as cells are resuspended they are checked for
agglutination