Assays for G6PD - 1
Fluorescent screening method:
Following
reagents placed on filter paper and patient’s hemolysate
added. Amount of fluorescence is directly proportional
to amount of G6PD present in serum
NADP
Glucose-6-phosphate
Reaction
of assay:
Glucose-6-phosphate
+
NADP
G6PD
6-phosphogluconate
+
NADPH
Non-fluorescent
fluorescent
Fluorescence
observed using Wood’s lamp