Assays for G6PD - 1
Quantitative method:
Following
reagents placed in test tube and patient’s hemolysate
added. Amount of light absorbed is directly proportional
to amount of G6PD present in serum
NADP
Glucose-6-phosphate
Reaction
of assay:
Glucose-6-phosphate
+
NADP
G6PD
6-phosphogluconate
+
NADPH
Absorbs light
Amount
of light absorbed is measured at 340 nm using spectrophotometer