Lab Assays for G6PD
Miscellaneous assays:
Methemoglobin
reducatase assay
G6PD
deficient red cells fail to reduce methemoglobin in the presence
of methylene blue.
Ascorbate-cyanide
assay
Ascorbate
+ cyanide +O2
Cyanide
prevents red cell catalase from breaking down H2O2
H2O2
Patient
hemolysate
methemoglobin
G6PD
deficient red cell solution turns brown (methb)
G6PD
normal red cell solution remains red (hb)
Not
specific for G6PD (PK and unstable Hbs positive)