Assays for PK - 1
Quantitative method:
Following
reagents placed in test tube:
ADP
Phosphoenolpyruvate
acid
Reaction
of assay:
ADP
+
ATP
PK
Pyruvic
acid
+
NADP
Pyruvic
acid
Decrease
in amount of light absorbed is measured at 340
nm using spectrophotometer
NADH
Phosphoenolpyruvate
acid
+
LD
lactic
acid
+
NAD