Assays for PK - 1
Screening method:
Following
reagents placed filter paper:
ADP
Phosphoenolpyruvate
acid
Reaction
of assay:
ADP
+
ATP
PK
Pyruvic
acid
+
NADP
Pyruvic
acid
Decrease
in amount of fluorescence is measured using Wood’s lamp.
NADH
Phosphoenolpyruvate
acid
+
LD
lactic
acid
+
NAD
Non-fluorescent
fluorescent