Confirmation of Negative DAT
When the DAT is negative it must be confirmed by
adding
“check cells”.
Check cells are red
blood cells that have had antibodies (IgG) attached to them.
If visible
agglutination occurs it confirms that the AHG has not been
neutralized and that the DAT is truly negative.
If the red cells do
not agglutinate it indicates the AHG was not working and either new AHG is needed
or
the patient’s cells need to be washed more than 3 times.
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