Hb F - 1
Quantitation
by alkali denaturation:
Most
adult hemoglobins are denatured when exposed to strong alkaline
solutions but Hb F is not. When ammonium sulfate is
added it stopps the denaturation process and
precipitates the denatured hemoglobins. Hb F
remains in solution and can then be measured.
Quantitation
by acid elution: (betke-Kleihauer)
Adult
hemoglobins are eluted from red cells when
exposed to strong acid solutions but Hb F is not.
The red cells are then stained and the number
of Hb F containing red cells can then be determined