Direct-Immunocytochemical Procedure
Principle:
Conjugated-antibody
reacts with antigen on cell or tissue.
If
it is an enzyme-conjugated antibody then a chromogen
is added so reaction between conjugated-antibody and
antigen can be visualized.
If
it is a fluorochrome-conjugated antibody then reaction
can be measured or visualized by fluorescence produced.
Pros:
Easy
to perform
Cons:
Least
sensitive and difficult to interpret results