Indirect-Immunocytochemical Procedure 2-step
Principle:
Unconjugated-antibody
reacts with antigen on cell or tissue.
A
conjugated-anti-immunoglobulin antibody is added.
Depending
on the tag used the reaction between the antibodies and antigen is
measured or visualized.
Pros:
More
difficult to perform
Cons:
sensitive
and easier to interpret results