Indirect-Immunocytochemical Procedure 3-step
Principle:
Unconjugated-antibody
reacts with antigen on cell or tissue.
A
conjugated-anti-immunoglobulin antibody is added.
Depending
on the tag used the reaction between the antibodies and antigen is
measured or visualized.
Pros:
More
difficult to perform
Cons:
Increased
sensitivity
A 2nd-conjugated-anti-immunoglobulin
antibody is added which increases amount of tag..