Unlabeled-Immunocytochemical Procedure
Principle:
Uses
an enzyme plus an antibody specific for the enzyme
Both
the enzyme-antibody complex and the primary antibody must be
produced in the same animal.
Two methods:
Alkaline
phosphatase – anti-alkaline phosphatase (APAAP)
Peroxidase-Antiperoxidase
method (PAP)
If
they are different the secondary antibody will not
bind them together.