Chromosome Identification
Principle:
Q
banding is formed by fluorochrome dye binding with A-T
rich areas
GTG
banding is formed by pre-treating with trypsin then staining
with Giemsa stain:
G+ bands =
A-T
rich areas stain heavy
G- bands =
G-C
rich areas stain poorly
R
banding is formed by pre-treating with hot alkali then staining
with Giemsa stain:
G+ bands =
A-T
rich areas stain poorly
R bands =
G-C
rich areas stain heavily
C
banding is formed by pre-treating with acid then with hot
alkali then staining with Giemsa stain:
The centromeres
(primary constriction) stains