Cytochemical Classification-2
a-naphthyl
acetate (also called NSE):
Monocytes
positive
Esterases (depends on substrates used):
Monocytes
reactivity is inhibited if NaF added
a-naphthyl
butyrate (also called NSE):
Monocytes
positive
Periodic Acid-Schiff test:
Positive
in all cells
Differentiation
is based on pattern of reactivity:
Granulocytes:
Strongly positive
Megakaryocytes:
Platelets:
ALL:
Diffusely positive
Intense red-pink
positive
Course block pattern
positive
Fine diffuse pattern
positive
Combination of both or
negative