Analytic Methods
Forward light
scatter:
Measures
size of cell
Side scatter
Measures
cell density
Use of fluorochromes attached to monoclonal antibodies that are specific for certain cell
structures
Some
common fluorochromes used:
Extrinsic cellular characteristics measured by:
Measures
cell complexity
Measures
cell granularity
Fluorescein isothiocyanate:
Phycoerythrin:
Peridinin chlorophyll protein:
Green
Red
Yellow-red