Electronic Impedance-2
Factors
influencing count:
Size
of aperture:
Protein buildup decreases size of aperture
Falsely increases size of cells
Passage
of more than one cell through aperture at a time:
Falsely increases cell size
Falsely lowers number of cells
Coincidence:
Falsely decreases cell number
Hydrodynamic
focusing:
Sample
stream is surrounded by sheath fluid which:
Focuses sample in middle of aperture
Prevents buildup of protein
Reduces coincidence