Optical Scatter - 1
Principle:
A hydrodynamically focused sample stream is directed through a quartz flow cell
past a focused light source (tungsten-halogen or helium-neon laser) and the cell causes light to
be scattered.
Measures cell volume
Measures internal complexity
Forward
light scatter (0o):
Side
light scatter (90o):
Light scatter measured by following detectors:
Forward-low
angle scatter (2-3o):
Measures cell volume
Forward-high
angle scatter (5-15o):
Measures internal complexity