Specimen Requirements
Anticoagulant
used:
3.2%
or 0.105 – 0.109 M buffered sodium citrate
Tube requirements:
Plastic
tubes preferred
Glass
tubes if coated with silicone
Reduces factor/plt activation
Order of tube filling:
Clot
tubes first – no additives
Sodium
citrate next
Other
tubes with additives (EDTA, heparin etc)
Ratio of blood to sodium citrate:
9
parts blood to 1 part sodium citrate (9:1)
Must
be within 90% of 9:1 ratio
Click here to see calculations:
