aPTT
– 1
Phospholipid – substitute for PF3
Reagents:
Negatively charged activator:
kaolin
elagic acid
0.025 M CaCl2
celite
Procedure:
(samples run in duplicate)
Activator + phospholipid added to
patient’s PPP
Incubation of 3 minutes :
Factors up through IX
of intrinsic pathway activated
CaCl2 added to start timer
Timer stopped when clot detected
Click on computer to
see cascade theory --
